Regulatory

Part:BBa_K2066126:Design

Designed by: Kalen Clifton, Christine Gao, Andrew Halleran, Ethan Jones, Likhitha Kolla, Joseph Maniaci, John Marken, John Mitchell, Callan Monette, Adam Reiss   Group: iGEM16_William_and_Mary   (2016-10-14)


Synthetic Enhancer Project: NRII2302 Upstream Regulatory Region with Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The plasmid maps we received from Amit et al. were unclear as to the location of the promoter versus the location of upstream spacer, as they only listed the start of transcription. However, it was known that this sequence was derived from the endogenous nitrogen regulation promoter, so we took, a significant enough amount so as to be certain to capture the promoter. Since a single NRII protein can phosphorylate many NRI proteins, any weakly constitutive promoter should be sufficient.

Source

Amit et al. 2012

References